產(chǎn)品名稱 |
Reovirus 3 |
商品貨號(hào) |
B243062 |
Classification |
Reoviridae, Orthoreovirus, Mammalian orthoreovirus |
Deposited As |
Reovirus type 3 |
Agent |
Reovirus 3 |
Strain |
Dearing |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Hemagglutinates with RBC (Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038).
|
Effect on Host |
CPE, granulation and sloughing |
Recommended Host |
LLC-MK2 Derivative (ATCC® CCL-7.1™)
|
Growth Conditions |
Temperature: 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:
3-5 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
Name of Depositor |
WA Chappell |
Source |
Child with diarrhea |
References |
Ramos-Alvarez M, Sabin AB. Enteropathogenic viruses and bacteria; role in summer diarrheal diseases of infancy and early childhood. J. Am. Med. Assoc. 167: 147-156, 1958. PubMed: 13538681
Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038
|
Cross References |
Nucleotide (GenBank) :
AJ133091
Reovirus type 3 S2 gene, strain Dearing.
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