Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.1, 0.1; PstI--3.05, 1.05, 0.1; XbaI/HindIII--3.0, 1.2, 0.1; Bgl--2.3, 1.3, 0.7; BamHI--3.05, 1.15, 0.2. The insert contains the following restriction sites (approximate kb from the 5' end): Pst--0.06, 0.23; BglI--0.47; SacI--0.73; XhoI--0.74; BamHI--1.0; SphI--1.03; HindIII--1.11. Contains the complete coding sequence, nt 1218-2462 of the sequence record. Transcription from the T7 promoter gives the sense strand. |
References |
Miller DA, et al. Murine transforming growth factor-beta2 cDNA sequence and expression in adult tissues and embryos. Mol. Endocrinol. 3: 1108-1114, 1989. PubMed: 2797004
Su. Wen Qian, personal communication
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