Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--11.5, 3.4, 2.3; BamHI--17.0; HindIII--7.8, 4.4, 2.5, 1.1, 0.4; PstI--12.0, 4.7. The insert contains the following restriction sites (approximate kb from the 5' XhoII site): EcoRI--2.8, 5.0; HindIII--8.1, 10.6, 11.05, 12.05; NsiI--8.0; PstI--1.8; EcoRV--5.2; NcoI--11.8; SpeI--2.9, 5.5, 9.7; XhaII--2.1. Distributed in aliquots of 250 ng (10 ng/ul). |