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Gallid herpesvirus 3
Gallid herpesvirus 3
規(guī)格:
貨期:
編號(hào):B163137
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Gallid herpesvirus 3
商品貨號(hào) B163137
Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus type 2
Agent Gallid herpesvirus 3
Strain SB-1 (clone of SB)
Applications
Will protect against MDV and non-virus producing JM tumor transplant.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
chicken (S-strain) peripheral blood lymphocytes
Product Format frozen
Storage Conditions ?Vapor phase of liquid nitrogen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
Non-oncogenic, cell-associated. Will protect against MDV and non-virus producing JM tumor transplant.
Note that SB-1 is strictly cell-associated, damage to the infected cells will cause loss of titer. Thaw frozen ampule in 27C waterbath; dilute content slowly in 9.0 ml medium or PBS; pellet cells (10min at 400Xg); resuspend in culture medium.
Effect on Host
?CPE, cell rounding and syncytia formation
Recommended Host
chicken embryo fibroblasts
Growth Conditions
Temperature: 38°C to 39°C
Incubation: ?Incubate infected culture for 7 days at 38-39°C in a humidified 5% CO2 atmosphere, until CPE are well advance throughout.

Cryopreservation
Storage temperature: Liquid nitrogen
Name of Depositor BW Calnek
U.S. Patent
U.S. Patent Number
Source
chicken (S-strain) peripheral blood lymphocytes
References

Schat KA, Calnek BW. Characterization of an apparently nononcogenic Marek's disease. J. Natl. Cancer Inst. 60: 1075-1082, 1978. PubMed: 76680

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