產品名稱 |
Canine distemper virus |
商品貨號 |
B163006 |
Classification |
Paramyxoviridae, Morbillivirus |
Deposited As |
Canine distemper |
Agent |
Canine distemper virus |
Strain |
Lederle |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
|
Comments |
Belongs to the same virus subgroup as measles and rinderpest. |
Effect on Host |
CPE, refractile cell rounding and degeneration |
Recommended Host |
SL-29 (ATCC® CRL-1590™) |
Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-8 days. Replace virus growth medium every 2-3 days. |
Name of Depositor |
VJ Cabasso, PJ Vasington, Lederle Laboratories Division of American Cyanamid Company |
Source |
Field case of distemper encephalitis in dog |
References |
Cabasso VJ, Cox HR. A distemper-like strain of virus derived from a case of canine encephalitis: its adaptation to the chick embryo and subsequent modification. Cornell Vet. 42: 96-107, 1952. PubMed: 14905890
|