產(chǎn)品名稱 |
Human adenovirus 42 |
商品貨號(hào) |
B162976 |
Classification |
Adenoviridae, Mastadenovirus, Human adenovirus D |
Agent |
Human adenovirus 42 |
Strain |
54/82 |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
|
Comments |
No cross-reactions by serum neutralization. Significant cross-reactions with Human adenovirus 15 and 22, bilaterally, by hemagglutinin inhibition assay. Hemagglutinates human or rhesus red blood cells. VR-1792™ was derived from VR-1304™ following treatment with diethyl ether to remove mycoplasma contamination. |
Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
HEp-2 (ATCC® CCL-23™) |
Growth Conditions |
Temperature: 36°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.05). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
Name of Depositor |
JC Hierholzer, Centers for Disease Control and Prevention; ATCC |
Source |
Stool from an asymptomatic 1 year old male, Paris, 1982. Treated to remove mycoplasma at ATCC. |
References |
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016
Wigand R, et al. A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch. Virol. 94: 283-286, 1987. PubMed: 3034212
|