| Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
| Component |
| Type Strain |
Volume |
| Biosafety Level |
Composition |
| Product Format |
Storage |
| Storage Conditions |
Lysis Buffer |
| 梅經(jīng)理 |
17280875617 |
1438578920 |
| 胡經(jīng)理 |
13345964880 |
2438244627 |
| 周經(jīng)理 |
17757487661 |
1296385441 |
| 于經(jīng)理 |
18067160830 |
2088210172 |
| 沈經(jīng)理 |
19548299266 |
2662369050 |
| 李經(jīng)理 |
13626845108 |
972239479 |
会理县|
延寿县|
南召县|
汉中市|
常宁市|
顺平县|
吉安市|
镇巴县|
高清|
汉寿县|
塔河县|
六枝特区|
孙吴县|
茂名市|
句容市|
衡阳县|
剑川县|
大名县|
三河市|
亚东县|
鲁山县|
金湖县|
保山市|
德兴市|
成都市|
陆良县|
凯里市|
策勒县|
九江县|
潼南县|
同仁县|
平舆县|
克什克腾旗|
西和县|
嘉定区|
泰州市|
上栗县|
丁青县|
蓬莱市|
万荣县|
哈巴河县|
|
